Overcoming the limitations of COVID-19 diagnostics with nanostructures, nucleic acid engineering, and additive manufacturing

Nantao Li, Bin Zhao, Robert Stavins, Ana Sol Peinetti, Neha Chauhan, Rashid Bashir, Brian T. Cunningham, William P. King, Yi Lu, Xing Wang, Enrique Valera

Current Opinion in Solid State and Materials Science 26 (2022) 100966

The COVID-19 pandemic revealed fundamental limitations in the current model for infectious disease diagnosis and serology, based upon complex assay workflows, laboratory-based instrumentation, and expensive materials for managing samples and reagents. The lengthy time delays required to obtain test results, the high cost of goldstandard PCR tests, and poor sensitivity of rapid point-of-care tests contributed directly to society’s inability to efficiently identify COVID-19-positive individuals for quarantine, which in turn continues to impact return to normal activities throughout the economy. Over the past year, enormous resources have been invested to develop more effective rapid tests and laboratory tests with greater throughput, yet the vast majority of engineering and chemistry approaches are merely incremental improvements to existing methods for nucleic acid amplification, lateral flow test strips, and enzymatic amplification assays for protein-based biomarkers. Meanwhile, widespread commercial availability of new test kits continues to be hampered by the cost and time required to develop single-use disposable microfluidic plastic cartridges manufactured by injection molding


Enzymes hosted in redox-active ionically cross-linked polyelectrolyte networks enable more efficient biofuel cells

Lucy L Coria-Oriundo, M Lorena Cortez, Omar Azzaroni, Fernando Battaglini

Soft Matter, 2021, 17, 5240-5247

Redox mediators are pivotal players in the electron transfer process between enzymes and electrodes. We present an alternative approach for redox mediation based on branched polyethyleneimine (BPEI) modified with an osmium complex. This redox polyelectrolyte is crosslinked with phosphate to produce colloidal particles with a diameter of ca. 1 μm, which, combined with glucose oxidase (GOx), can form electroactive assemblies through either layer by layer assembly (LbL) or one-pot drop-casting (OPDC). The addition of NaCl to these colloidal systems induces the formation of films that otherwise poorly grow, presenting an outstanding catalytic current. The system was tested as a bioanode delivering a power output of 148 μW per nmol of mediator. These results are explained in terms of the interactions of the ions with the polyelectrolyte and represent a new route for the development of bioelectrochemical devices involving redox mediators and enzymes.


Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system

Lucy L Coria-Oriundo, Fernando Battaglini, Sonia Wirth

Ecotoxicology and Environmental Safety, 217, 112237 (2021)

Laccases and laccase-mediator systems (LMS) are versatile catalysts that can oxidize a broad range of substrates coupled to the sole reduction of dioxygen to water. They possess many biotechnological applications in paper, textile, and food industries, bioethanol production, organic synthesis, detection and degradation of pollutants, and biofuel cell development. In particular, bacterial laccases are getting relevance due to their activity in a wide range of pH and temperature and their robustness under harsh conditions. However, the enzyme and the redox mediator's availability and costs limit their large-scale commercial use. Here we demonstrate that β-(10-phenothiazyl)-propionic acid can be used as an efficient and low-cost redox mediator for decolorizing synthetic dyes by the recombinant laccase SilA from Streptomyces ipomoeae produced in E. coli. This new LMS can decolorize more than 80% indigo carmine and malachite green in 1 h at pH = 8.0 and 2 h in tap water (pH = 6.8). Furthermore, it decolorized more than 40% of anthraquinone dye remazol brilliant blue R and 80% of azo dye xylidine ponceau in 5 h at 50 °C, pH 8.0. It supported at least 3 decolorization cycles without losing activity, representing an attractive candidate for a cost-effective and environmentally friendly LMS functional at neutral to alkaline pH.


Functionalized hierarchical wrinkled-silica spheres for laccases immobilization

Keyla M Fuentes, Lucy L Coria-Oriundo, Sonia Wirth, Sara A Bilmes

Journal of Porous Materials 28, 261-269 (2021)

Functionalized mesoporous SiO2 are common supports for some enzymes of industrial interest, such as laccases. However, the incorporation of specific functionalities and the loading of enzymes with dimensions close to the diameters of the pores obstructs the porous system. For biotechnological applications, tailored porous supports are still needed to enhance the laccase loading. The hierarchical meso/macroporous system in wrinkled-SiO2 spheres (w-SiO2) is a suitable option to overcome this issue. Herein, (3-aminopropyl) triethoxysilane and glutaraldehyde were use as functionalizing agents for the immobilization of laccase on w-SiO2. The functionalization occurs in the mesopores of the wrinkled walls and the preservation of the macroporous entries facilitates the diffusion of the laccase inside the particle. The enzyme performance was evaluated by means of the crystal violet bleaching. The enzyme is stabilized through the imine groups provided by glutaraldehyde, allowing the retention of the activity after several reaction cycles. The bleaching can be boosted by acetosyringone, highlighting the possibility of using redox mediators to expand the range of oxidizable substrates. Understanding the effect of w-SiO2 functionalization on laccases loading and performance could be extrapolated to other enzymes with biotechnological interest that requires this type of hierarchical porous silica.


Mass and charge transport in highly mesostructured polyelectrolyte/electroactive-surfactant multilayer films

Esteban Piccinini, Graciela A González, Omar Azzaroni, Fernando Battaglini

Journal of Colloid and Interface Science 581, 595-607,2021

The assembly of polyacrylic acid and different combinations of redox-modified and unmodified hexadecyltrimethylammonium bromide yields highly structured and electroactive thin films. The growth, viscoelastic properties, mass, and electron transport of these films were studied by combining electrochemical and quartz crystal balance with dissipation experiments.


Redox Polyelectrolyte Modified Gold Nanoparticles Enhance the Detection of Adenosine in an Electrochemical Split-Aptamer Assay

Lucy L Coria-Oriundo, Helena Ceretti, Yoann Roupioz, Fernando Battaglini

ChemistrySelect, 5(36), 11391-11398

We present an electrochemical sandwich-type assay based on the splitting of an aptamer into two fragments. Gold nanoparticles are modified with one of the fragments and a redox polyelectrolyte. The first is used as the recognition element, while the other for the electrochemical signal generation. The split-aptamer used here can detect adenosine, used as a model system for recognizing small molecules. The multiple binding sites on the nanoparticle, along with the high number of redox probes, yield a selective and sensitive assay for adenosine, achieving a limit of detection of 3.1 nM and a linear range up to 75 nM. The obtained results are analyzed in terms of the nanoparticle and electrode architectures. The assay can be easily extended to other small molecules and sandwich assays, representing a promising tool for detecting metabolites at the nanomolar level.


Mesostructured Electroactive Thin Films Through Layer-by-Layer Assembly of Redox Surfactants and Polyelectrolytes

Esteban Piccinini, Marcelo Ceolín, Fernando Battaglini, Omar Azzaroni

ChemPlusChem 85-8,1616-1622,2020

The layer‐by‐layer assembly of polyelectroIMlytes and surfactants enables structural control of multilayers displaying meso‐organized 3D redox‐active domains. The approach exploits the dual role of surfactants as functional units, i. e.: redox active centers, and structural units, such as structure‐directing agents. The population of redox centers hosted into the film can be manipulated by co‐assembling electroactive and non‐electroactive surfactants. This strategy constitutes a straightforward technique to produce electroactive films with precise control over the spatial organization of their redox domains, even under dynamic electrochemical conditions.


Flexible conducting platforms based on PEDOT and graphite nanosheets for electrochemical biosensing applications

Juliana Scotto, Esteban Piccinini, Catalina von Bilderling, Lucy L Coria-Oriundo, Fernando Battaglini, Wolfgang Knoll, Waldemar A Marmisolle, Omar Azzaroni

Applied Surface Science, 146440, 2020

In this work, we present a novel strategy for producing graphite nanosheets (GNS) dispersed in the solvent employed then for the chemical synthesis of PEDOT, which allows producing composite nanofilms on plastic substrates for the construction of transparent and flexible all-polymer electrodes. By an optimized experimental procedure, we achieved a proper integration of PEDOT and GNS within ultrathin (<100 nm) composite films and good enough conductivity to ensure adequate electrochemical response without the requirement of conducting base electrodes. We tested the performance of these platforms for real applications by developing glucose biosensors by molecular integration of supramolecular assembly of glucose oxidase and an electroactive polyelectrolyte on top of the PEDOT-GNS coatings.


Portable microalgal biosensor for herbicide monitoring

Ignacio Boron, Angela Juarez, Fernando Battaglini


biosensor composed by unicellular microalgae hosted in a mechanically stable and electrochemically sensitive material is cast on a screen‐printed electrode and mounted in a flow‐cell system. The biosensor presents a limit of detection of 24 ppb to atrazine and a long‐term stability, making this system suitable for remote sensing.


Silver-Assisted Synthesis of High-Indexed Palladium Tetrahexahedral Nanoparticles and Their Morphological Variants

Nitya Sai Reddy Satyavolu, Ana Sol Peinetti, Yiming Wang, Arzeena Sultana Ali, Jeffrey Wayjer Lin, Yi Lu

Chemistry of Materials 31 (8), 2923-2929, 2019

This study establishes a synthetic procedure that is scalable and is both chemically and economically more accessible than the existing protocols for Pd THH nanoparticles, making it possible for much wider applications of the Pd THH nanoparticles and its variants. Finally, these particles displayed higher electrocatalytic activity for ethanol oxidation reaction compared to low-indexed faceted Pd nanoparticles and commercially available Pd catalysts.


DNAzyme-based biosensor as a rapid and accurate verification tool to complement simultaneous enzyme-based media for E. coli detection

Noor Zaouri, Zhengfang Cui, Ana Sol Peinetti, Yi Lu, Pei-Ying Hong

Environmental Science: Water Research & Technology 5 (12), 2260-2268, 2019

This study aimed to achieve rapid verification test of E. coli using DNAzyme-based biosensor. The biosensor was first tested for four E. coli strains against more than 90 non-E. coli strains routinely causing false positive results and showed high specificity towards E. coli strains. The biosensor was then determined for its detection sensitivity. It was observed that a single E. coli colony would generate a robust fluorescence signal after 2 h of sub-culturing. With this short incubation time, non-E. coli strains were unable to generate any apparent fluorescence signal, hence further improving the detection specificity of biosensor. Lastly, we further demonstrate that the biosensor can be applied to different commercial brands of simultaneous enzyme-based agar to consistently identify the presence of E. coli. The biosensor was able to rapidly denote presence of discrete E. coli colonies by binding on to protein targets possibly of DNA replication or motility functions that were predominantly specific to E. coli.


The effect of ionic strength and phosphate ions on the construction of redox polyelectrolyte–enzyme self-assemblies

Daniele Zappi, Lucy L Coria-Oriundo, Esteban Piccinini, Marcos Gramajo, Catalina von Bilderling, Lía I Pietrasanta, Omar Azzaroni, Fernando Battaglini

Physical Chemistry Chemical Physics 21 (41), 22947-22954, 2019

Our results show that the environment from which the assembly is constructed has a significant effect on the electrochemical response. Notably, the polyelectrolyte dissolved in the presence of phosphate at high ionic strength presents a globular structure which is preserved after adsorption with substantial effects on the buildup of the multilayer system, improving the electron transfer process through the film.


Disposable Electrochemical Sensor for Rapid Determination of Ethyl Xanthate in the Mining Industry

Ignacio Pedre, Fernando Battaglini, Graciela A González

Electroanalysis 30 (11), 2589-2596, 2019

A simple and reliable method is presented for the direct monitoring of xanthate in flotation baths and their effluents, as a tool for their reconditioning, and reducing their environmental impact. For this purpose, screen‐printed graphite electrodes (SPE) were modified with a polyelectrolyte‐surfactant matrix which was able to adsorb the xanthate, whose concentration is determined by electrochemical impedance spectroscopy (EIS) following the change in the electron transfer resistance of ferrocyanide after a pre‐oxidation treatment.


Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array

Ana S Peinetti, Helena Ceretti, Martín Mizrahi, Graciela A González, Silvana A Ramírez, Félix G Requejo, Javier M Montserrat, Fernando Battaglini

Bioelectrochemistry 121, 169-175, 2018

Our results prove that low DNA coverages on the confined nanoparticles prompt to a more sensitive response, showing the relevance in avoiding the DNA strand overcrowding. The system was able to determine a concentration as low as 100 pM of the complementary strand, thus introducing the foundations for the construction of label-free genosensors at the nanometer scale.


Highly sensitive biosensing with solid-state nanopores displaying enzymatically reconfigurable rectification properties

Gonzalo Pérez-Mitta, Ana S Peinetti, M Lorena Cortez, María Eugenia Toimil-Molares, Christina Trautmann, Omar Azzaroni

Nano Letters 18 (5), 3303-3310, 2018

In this work, we describe the construction of novel ultrasensitive enzymatic nanopore biosensors employing “reactive signal amplifiers” as key elements coupled to the transduction mechanism. The proposed framework offers innovative design concepts not only to amplify the detected ionic signal and develop ultrasensitive nanopore-based sensors but also to construct nanofluidic diodes displaying specific chemo-reversible rectification properties. The integrated approach is demonstrated by electrostatically assembling poly(allylamine) on the anionic pore walls followed by the assembly of urease.


Synthesis of nickel entities: from highly stable zerovalent nanoclusters to nanowires. Growth control and catalytic behavior

Ana S Peinetti, Martín Mizrahi, Félix G Requejo, David Buceta, M Arturo López-Quintela, Graciela A González, Fernando Battaglini

Journal of colloid and interface science 516, 371-378, 2018

Synthesis of low atomicity Ni nanoclusters is carried out in 10 nm diameter mesoporous alumina allowing the production of zerovalent ligand-free Ni nanoclusters estimated in 13 atoms. The size and shape of the Nickel entities inside the pores can be controlled by the current density applied during the reduction process. The nanoclusters show a superior performance as catalysts for the reduction of methylene blue.


3-D Architectures for Bioelectrochemistry

Fernando Battaglini, Ana S Peinetti

Encyclopedia of Interfacial Chemistry. Surface Science and Electrochemistry, 2018


Surfactants as mesogenic agents in layer-by-layer assembled polyelectrolyte/surfactant multilayers: nanoarchitectured “soft” thin films displaying a tailored mesostructure

Esteban Piccinini, Jimena S Tuninetti, Joseba Irigoyen Otamendi, Sergio E Moya, Marcelo Ceolín, Fernando Battaglini, Omar Azzaroni

Physical Chemistry Chemical Physics 20 (14), 9298-9308, 2018

. In the present work we demonstrate that the integration of surfactants (as mesogenic agents) into layer-by-layer (LbL) assembled polyelectrolyte multilayers offers a straightforward approach to control the internal film organization at the mesoscale level. The mesostructure of films constituted of hexadecyltrimethylammonium bromide, CTAB, and polyacrylic acid, PAA (of different molecular weights), was characterized as a function of the number of assembled layers.


Highly-organized stacked multilayers via layer-by-layer assembly of lipid-like surfactants and polyelectrolytes. Stratified supramolecular structures for (bio)electrochemical nanoarchitectonics

M Lorena Cortez, Agustín Lorenzo, Waldemar A Marmisollé, Catalina Von Bilderling, Eliana Maza, Lía Pietrasanta, Fernando Battaglini, Marcelo Ceolín, Omar Azzaroni

Soft Matter 14 (10), 1939-1952, 2018

Herein, we report on the facile preparation and application of highly organized stacked multilayers via layer-by-layer assembly of lipid-like surfactants and polyelectrolytes. Polyelectrolyte multilayers with high degree of stratification of the internal structure were constructed through consecutive assembly of polyallylamine and dodecyl phosphate, a lipid-like surfactant that act as a structure-directing agent.


Integration of biorecognition elements on PEDOT platforms through supramolecular interactions

Luciano D Sappia, Esteban Piccinini, Waldemar Marmisollé, Natalia Santilli, Eliana Maza, Sergio Moya, Fernando Battaglini, Rossana E Madrid, Omar Azzaroni

Advanced Materials Interfaces 4 (17), 1700502, 2017

Biorecognizable carbohydrate motifs are integrated onto poly(3,4‐ethylenedioxythiophene) (PEDOT) films through the preparation poly(allylamine)–PEDOT composites via supramolecular interactions. The proposed strategy facilitates the straightforward supramolecular construction of versatile all‐plastic bioelectrochemical platforms. The bioelectrocatalytic detection of glucose is studied. Results reveal that the use of supramolecular PEDOT platforms leads to an enhancement of the bioelectrocatalytic signal.


Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications

Esteban Piccinini, Christina Bliem, Ciril Reiner-Rozman, Fernando Battaglini, Omar Azzaroni, Wolfgang Knoll

Biosensors and Bioelectronics 92, 661-667, 2017

We present the construction of layer-by-layer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect transistors (rGO FETs) for the detection of urea. This versatile biosensor platform simultaneously exploits the pH dependency of liquid-gated graphene-based transistors and the change in the local pH produced by the catalyzed hydrolysis of urea.


Versatile Electrochemical Platform for the Determination of Phenol‐like Compounds Based on Laccases from Different Origins

Ignacio Borón, Sonia Wirth, Fernando Battaglini

Electroanalysis 29 (2), 616-621, 2017

Through adequate laccase immobilization, disposable screen printed electrodes can be used as interphase to build amperometric phenol sensors. In this work three different laccases were studied for the determination of phenol‐like compounds, two of them are isoenzymes from Trametes trogii and the third one from Rhus vernicifera. Their immobilization on screen printed electrodes is presented for the construction of amperometric sensors. The electrode substrate is composed by graphite screen printed electrodes modified with carbon nanotubes and silica microspheres where, depending on the application, one of the three laccases is adsorbed.


Solvent Effects on the Structure–Property Relationship of Redox-Active Self-Assembled Nanoparticle–Polyelectrolyte–Surfactant Composite Thin Films: Implications for the Generation of Bioelectrocatalytic Signals in Enzyme-Containing Assemblies

M Lorena Cortez, Marcelo Ceolín, Luis Cuellar Camacho, Edwin Donath, Sergio E Moya, Fernando Battaglini, Omar Azzaroni

ACS applied materials & interfaces 9 (1), 1119-1128, 2017

Our studies reveal that the solvent used in processing the supramolecular films and the presence of metal nanoparticles not only have a substantial influence in determining the mesoscale organization and morphological characteristics of the film but also have a strong influence on the efficiency and performance of the bioelectrochemical system. In particular, a higher bioelectrochemical response is observed when nanocomposite supramolecular films were cast from aqueous solutions. These observations seem to be associated with the fact that the use of aqueous solvents increases the hydrophilicity of the film, thus favoring the access of glucose, particularly at low concentrations.


Electrochemical sensor for thiourea focused on metallurgical applications of copper

Ignacio Pedre, L Méndez DeLeo, Maria Guadalupe Sánchez-Loredo, Fernando Battaglini, Graciela Alicia González

Sensors and Actuators B: Chemical 232 ,383-389 ,2016

A simple and reliable device is presented for the direct monitoring of thiourea in copper electrorefinery baths as a tool for reconditioning of industrial baths, and so reducing its environmental impact. For this purpose, a screen-printed graphite electrode is modified with silver nanoparticles (AgNPs) contained in a polyelectrolyte-surfactant matrix.


Numerical simulation of the diffusion processes in nanoelectrode arrays using an axial neighbor symmetry approximation

Ana Sol Peinetti, Rodrigo S Gilardoni, Martín Mizrahi, Felix G Requejo, Graciela A González, Fernando Battaglini

Analytical chemistry 88 (11), 5752-5759, 2016

In this work, we present a computational model able to simulate the electrochemical behavior of arrays working either as the summation of individual electrodes or being affected by the overlapping of the diffusional fields without previous considerations. Our computational model relays in dividing a regular electrode array in cells. In each of them, there is a central electrode surrounded by neighbor electrodes; these neighbor electrodes are transformed in a ring maintaining the same active electrode area than the summation of the closest neighbor electrodes.


Self-limited self-assembly of nanoparticles into supraparticles: towards supramolecular colloidal materials by design

Esteban Piccinini, Diego Pallarola, Fernando Battaglini, Omar Azzaroni

Molecular Systems Design & Engineering 1 (2), 155-162 2016

For quite a while, scientists have resorted to colloidal synthesis to mimic complex structural and functional materials found in Nature. In particular, within the past few years, the synthesis of suprastructures with novel properties that emerge from the coupling of diverse nanoscale functional units has defined new boundaries in materials science. In this mini-review, we survey the most recent and outstanding achievements on the rational design of supraparticles based on the self-limited self-assembly of nanoparticles, and their application in fields like biology, medicine and energy.


Hydrophobic interactions leading to a complex interplay between bioelectrocatalytic properties and multilayer meso-organization in layer-by-layer assemblies

M.L. Cortez, N. de Matteis, M. Ceolín, W. Knoll, F. Battaglini, O. Azzaroni

Physical Chemistry Chemical Physics 16, 20844-20855, 2014


Formation of redox-active self-assembled polyelectrolyte–surfactant complexes integrating glucose oxidase on electrodes: Influence of the self-assembly solvent on the signal generation

M Lorena Cortez, Marcelo Ceolín, Omar Azzaroni, Fernando Battaglini

Bioelectrochemistry,105 ,117-122 , 2015

In this work the effects of the self-assembly solvent on the structure and electrochemical behavior of redox-active polyelectrolyte–surfactant complexes cast on electrode supports from aqueous and DMF solutions are presented. The complex studied is formed by complexation of osmium complex-modified polyallylamine (OsPA) with dodecyl sulfate (DS) surfactants.


Detection of thiourea from electrorefining baths using silver nanoparticles-based sensors

Ignacio Pedre, Fernando Battaglini, Gladis Judith Labrada Delgado, María Guadalupe Sánchez-Loredo, Graciela A González

Sensors and Actuators B: Chemical 211,515-522,2015

A novel and easy-to-use colorimetric thiourea (TU) sensor based on immobilized polyvinylpyrrolidone-stabilized silver nanoparticles (PVP-AgNPs) is reported. The new sensor is aimed for its direct application in copper electrorefinery baths. Quantitative analysis was carried out by adding different thiourea amounts into a synthetic bath and monitoring the absorption changes of the surface plasmon resonance band (SPRB) by UV–Vis spectrophotometry. A linear correlation between the absorbance change and thiourea concentration was obtained, ranging from 0.125 mg/L to 1 mg/L.


Recognition-driven assembly of self-limiting supramolecular protein nanoparticles displaying enzymatic activity

Esteban Piccinini, Diego Pallarola, Fernando Battaglini, Omar Azzaroni

Chemical Communications 51(79),14754-14757,2015

We report the recognition-driven assembly of self-limiting protein nanoparticles displaying enzymatic activity. Solution self-assembly of concanavalin A lectin and glycoenzyme glucose oxidase leads to the spontaneous formation of biocolloids with well-defined dimensions, narrow size distribution and remarkable stability. These biocolloids successfully recognize a glycosylated modified electrode retaining the enzyme activity.


Confined gold nanoparticles enhance the detection of small molecules in label-free impedance aptasensors

Ana S Peinetti, Helena Ceretti, Martín Mizrahi, Graciela A González, Silvana A Ramírez, Felix G Requejo, Javier M Montserrat, Fernando Battaglini

Nanoscale 7 (17), 7763-7769, 2015

Gold nanoparticles with a controlled size (about 2 nm) are electrogenerated in the alumina cavities, showing a fast electron transfer process toward ferrocyanide. These uncapped nanoparticles are easily modified with a thiol-containing aptamer for label-free detection of adenosine monophosphate by electrochemical impedance spectroscopy. Our results show that the use of a limited electrical conducting surface inside an insulating environment can be very sensitive to conformational changes, introducing a new approach to the detection of small molecules, exemplified here by the direct and selective detection of adenosine monophosphate at the nanomolar scale.


Self-Assembled Redox Polyelectrolyte-Surfactant Complexes: Nanostructure and Electron Transfer Characteristics of Supramolecular Films with Built-In Electroactive Chemical Functions

M Lorena Cortez, Graciela A González, Marcelo Ceolín, Omar Azzaroni, Fernando Battaglini

Electrochimica Acta 118,124-129, 2014


Effect of Gold Nanoparticles on the Structure and Electron‐Transfer Characteristics of Glucose Oxidase Redox Polyelectrolyte‐Surfactant Complexes

M Lorena Cortez, Waldemar Marmisollé, Diego Pallarola, Lía I Pietrasanta, Daniel H Murgida, Marcelo Ceolín, Omar Azzaroni, Fernando Battaglini

Chemistry–A European Journal 20 (41),13366-13374,2014




Synthesis of atomic metal clusters on nanoporous alumina

Ana Sol Peinetti, Santiago Herrera, Graciela A González, Fernando Battaglini

Chemical Communications 49 (96), 11317-11319, 2013



A polyelectrolyte–surfactant complex as support layer for membrane functionalization

Ana Sol Peinetti, Lucila P Méndez De Leo, Graciela A González, Fernando Battaglini

Journal of colloid and interface science 386 (1), 44-50, 2012


Ionic self-assembly of electroactive biorecognizable units: electrical contacting of redox glycoenzymes made easy

M. L. Cortez, D. Pallarola, M. Ceolín, O. Azzaroni, F. Battaglini

Chem. Commun., 48 (2012) 10868–10870


Recognition-driven layer-by-layer construction of multiprotein assemblies on surfaces: a biomolecular toolkit for building up chemoresponsive bioelectrochemical interfaces

D. Pallarola, C. von Bildering, L. I. Pietrasanta, N. Queralto, W. Knoll, F. Battaglini, O. Azzaroni.

Phys. Chem. Chem. Phys., 14 (2012) 11027–11039.


Rationally designed porous silicon as platform for optical biosensors

G. Priano, L.N. Acquaroli, L.C. Lasave, F. Battaglini, R.D. Arce, R.R. Koropecki

Thin Solid Films 520 (2012) 6434–6439.


Electrochemical sensing platform based on polyelectrolyte-surfactant supramolecular assemblies incorporating carbon nanotubes

M. L. Cortez, M. Ceolín, O. Azzaroni, F. Battaglini

Analytical Chemistry, 83 (2011) 8011-8018.


An Electroactive Versatile Matrix for the Construction of Sensors

M. L. Cortez, G. A. Gonzalez, F. Battaglini

Electroanalysis, 23 (2011) 156-160



Facile Glycoenzyme Wiring to Electrode Supports by Redox-Active Biosupramolecular Glue

D. Pallarola, N. Queralto, W. Knoll, O. Azzaroni, F. Battaglini

Chemistry, a european journal, 16 (2010) .13970-13975


Sequential Electrochemical Polymerization of Aniline and their Derivatives Showing Electrochemical Activity at Neutral pH

J. Yánez-Heras, G. A. Planes, F. Williams, C. A. Barbero, F. Battaglini.

Electroanalysis, 22 (2010) 2801-2808


Chelating electrodes as taste sensor for the trace assessment of metal ions

J. Yánez Heras, S.D. Rodríguez, R.M. Negri, F. Battaglini.

Sensors & Actuators: B. Chemical, 145 (2010) 726-733.


Redox-Active Concanavalin A: Synthesis, Characterization and Recognition-Directed Assembly of Interfacial Architectures for Bioelectronic Applications

D. Pallarola, N. Queralto,W. Knoll, M. Ceolín, O. Azzaroni, F. Battaglini

Langmuir, 26 (2010) 13684-13696


Supramolecular Assembly of Glucose Oxidase on Concanavalin A-Modified Gold Electrodes.

D. Pallarola, N. Queralto, F. Battaglini, O. Azzaroni

Phys. Chem. Chem. Phys., 12 (2010) 8071-8083.


Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin

J. Yánez Heras, D. Pallarola, F. Battaglini

Biosensors and Bioelectronics, 25 (2010) 2470-2476.


Modeling the electrochemical response of mesoporous materials toward its application to biomolecular detection

Ana Sol Peinetti, Graciela A González, Fernando Battaglini

Electroanalysis 22 (12), 1329-1336, 2011